Journal: Cell reports

Article Title: Early Seizure Activity Accelerates Depletion of Hippocampal Neural Stem Cells and Impairs Spatial Discrimination in an Alzheimer’s Disease Model

doi: 10.1016/j.celrep.2019.05.101

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse anti-Prox1 , PhosphoSolutions , Cat#: 1685-Prox1; RRID: AB_2492217.

Techniques: Recombinant, Imaging, Software, Microscopy

Journal: Journal of Anatomy

Article Title: Prox1 induces new lymphatic vessel formation and promotes nerve reconstruction in a mouse model of sciatic nerve crush injury

doi: 10.1111/joa.13247

Figure Lengend Snippet: Expression of Prospero Homeobox 1 (Prox1) in the sciatic nerve. (A) Control, (B) 7 days after sciatic nerve injury, and (C) 14 days after sciatic nerve injury. (D) Number of Prox1‐positive cells. Black arrows: Prox1‐positive nuclei. The Prox1‐positive nuclei were mostly observed in the tubular endothelial cells. The number of Prox1‐positive nuclei were significantly greater in the sciatic nerve at 7 days after injury, compared to that in the control group. No significant difference was observed between the control group and injury model at 14 days after injury. * P < 0.05.

Article Snippet: The sections were incubated in rabbit anti‐mouse Prox1 antibody (diluted 1:100; Boster Biological Technology) or goat anti‐mouse lymphatic vessel endothelial hyaluronan receptor 1 (LYVE‐1) antibody (diluted 1:100; R&D Systems) at 4°C for 24 h, followed by incubation in horseradish peroxidase‐labeled goat anti‐rabbit IgG or rabbit anti‐goat IgG.

Techniques: Expressing, Control

Journal: Journal of Anatomy

Article Title: Prox1 induces new lymphatic vessel formation and promotes nerve reconstruction in a mouse model of sciatic nerve crush injury

doi: 10.1111/joa.13247

Figure Lengend Snippet: Co‐localization of lymphatic vessel endothelial hyaluronan receptor 1 (LYVE‐1)‐positive lymphatic vessels with Prospero Homeobox 1 (Prox1)‐positive endothelial nuclei in the sciatic nerve at 7 days after injury. (A) LYVE‐1 (green), (B) Prox1 (red), and (C) LYVE‐1 + Prox1+ (merged image), showing co‐localization of lymphatic vessels with endothelial cells (yellow). White star indicates areas within the nerve membranes.

Article Snippet: The sections were incubated in rabbit anti‐mouse Prox1 antibody (diluted 1:100; Boster Biological Technology) or goat anti‐mouse lymphatic vessel endothelial hyaluronan receptor 1 (LYVE‐1) antibody (diluted 1:100; R&D Systems) at 4°C for 24 h, followed by incubation in horseradish peroxidase‐labeled goat anti‐rabbit IgG or rabbit anti‐goat IgG.

Techniques:

Journal: IBRO Neuroscience Reports

Article Title: Prospero-related homeobox protein 1 (Prox1) enhances myelin sheath regeneration in injured sciatic nerve

doi: 10.1016/j.ibneur.2025.09.011

Figure Lengend Snippet: Double IF staining of Prox1 (red) and MBP (green) with IF (×200) and their intensity profile obtained using ImageJ software, along an ideal straight line (white).

Article Snippet: Rabbit anti-Myelin basic protein/MBP Polyclonal antibody (Proteintech, USA, catalog # 10458–1-AP); Mouse anti-MBP tag Monoclonal antibody (Proteintech, USA, catalog # 66003–1-Ig); Anti-PROX1 Antibody (Boster Bio, China, catalog # A01985–1); DAB Chromogenic Substrate Reagent Kit (Blue) (Boster Bio, China, catalog # AR1025); Rabbit Anti-GAPDH (Jiangsu KeyGEN BioTECH, China, catalog # KGC6102–1); Luxol Fast Blue Myelin Stain Kit (Solarbio, China, G3240); KeygenMAX 3000 transfection reagent (Jiangsu KeyGEN BioTECH, China, catalog # KGA9705–1); PrimeScriptTM RT Master Mix (TaKaRa, Japan, catalog # RR036B); Olympus Inverted Microscope (OLYMPUS, Japan, catalog # IX51); ABI Stepone real-time PCR (Thermo Fisher, USA); Trans-Blot® TurboTM Transfer System (BIO-RAD, USA, catalog #1704150).

Techniques: Staining, Software

Journal: IBRO Neuroscience Reports

Article Title: Prospero-related homeobox protein 1 (Prox1) enhances myelin sheath regeneration in injured sciatic nerve

doi: 10.1016/j.ibneur.2025.09.011

Figure Lengend Snippet: (A) IHC images of Prox1 proteins in sciatic nerve tissue of control sciatic nerves and injured sciatic nerves on Day 3, 4, 5, 6, 7 and 14 post-injuries (×200) and the quantification of Prox1 proteins in top right panel (P value was normalized to control group, **** P < 0.0001, n = 5, One-Way ANOVA analysis was used). (B) Western blot and quantitative analysis of Prox1 protein expression in sciatic nerve tissue from control nerves and injured nerves at days 3, 4, 5, 6, 7, and 14 post-injury (**** P < 0.0001, n = 3, One-Way ANOVA analysis was used).

Article Snippet: Rabbit anti-Myelin basic protein/MBP Polyclonal antibody (Proteintech, USA, catalog # 10458–1-AP); Mouse anti-MBP tag Monoclonal antibody (Proteintech, USA, catalog # 66003–1-Ig); Anti-PROX1 Antibody (Boster Bio, China, catalog # A01985–1); DAB Chromogenic Substrate Reagent Kit (Blue) (Boster Bio, China, catalog # AR1025); Rabbit Anti-GAPDH (Jiangsu KeyGEN BioTECH, China, catalog # KGC6102–1); Luxol Fast Blue Myelin Stain Kit (Solarbio, China, G3240); KeygenMAX 3000 transfection reagent (Jiangsu KeyGEN BioTECH, China, catalog # KGA9705–1); PrimeScriptTM RT Master Mix (TaKaRa, Japan, catalog # RR036B); Olympus Inverted Microscope (OLYMPUS, Japan, catalog # IX51); ABI Stepone real-time PCR (Thermo Fisher, USA); Trans-Blot® TurboTM Transfer System (BIO-RAD, USA, catalog #1704150).

Techniques: Control, Western Blot, Expressing

Journal: IBRO Neuroscience Reports

Article Title: Prospero-related homeobox protein 1 (Prox1) enhances myelin sheath regeneration in injured sciatic nerve

doi: 10.1016/j.ibneur.2025.09.011

Figure Lengend Snippet: MBP protein level was significantly increased by PROX1 overexpression. (A) qPCR quantification of PROX1 overexpression, (B) WB images of MBP and GAPDH protein from cells transfected with NC, pcDNA-vector or pcDNA-prox1, (C) the quantification of (B). (**** P < 0.0001, n = 3, One-Way ANOVA analysis was used).

Article Snippet: Rabbit anti-Myelin basic protein/MBP Polyclonal antibody (Proteintech, USA, catalog # 10458–1-AP); Mouse anti-MBP tag Monoclonal antibody (Proteintech, USA, catalog # 66003–1-Ig); Anti-PROX1 Antibody (Boster Bio, China, catalog # A01985–1); DAB Chromogenic Substrate Reagent Kit (Blue) (Boster Bio, China, catalog # AR1025); Rabbit Anti-GAPDH (Jiangsu KeyGEN BioTECH, China, catalog # KGC6102–1); Luxol Fast Blue Myelin Stain Kit (Solarbio, China, G3240); KeygenMAX 3000 transfection reagent (Jiangsu KeyGEN BioTECH, China, catalog # KGA9705–1); PrimeScriptTM RT Master Mix (TaKaRa, Japan, catalog # RR036B); Olympus Inverted Microscope (OLYMPUS, Japan, catalog # IX51); ABI Stepone real-time PCR (Thermo Fisher, USA); Trans-Blot® TurboTM Transfer System (BIO-RAD, USA, catalog #1704150).

Techniques: Over Expression, Transfection, Plasmid Preparation